Contactin 1 antibody (AA 100-150)

Catalog No. ABIN571798

Product Details anti-Contactin 1 Antibody

Target: Contactin 1 (CNTN1)

Binding Specificity: AA 100-150

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Contactin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Rabbit antibody to Contactin 1

Specificity: Specific for contactin-1.

Cross-Reactivity: Human, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 100-150 of human contactin 1 conjugated to an immunogenic carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Contactin 1

Target: Contactin 1 (CNTN1)

Alternative Name: Contactin 1 (CNTN1 Products)

Background: FUNCTION: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. SUBUNIT: Monomer. Interacts with CNTNAP1 in cis form. Binds to the carbonic-anhydrase like domain of protein-tyrosine phosphatase zeta. Interacts with NOTCH1 and TNR. SUBCELLULAR LOCATION: Cell membrane, Lipid-anchor GPI-anchor. MISCELLANEOUS: F3 shares with L1 N-CAM MAG and other cell adhesion molecules from nervous tissue the L2/HNK-1 carbohydrate epitope. TISSUE SPECIFICITY: Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung pancreas kidney and skeletal muscle.

UniProt: Q12860