DLGAP1 antibody (AA 391-405)

Catalog No. ABIN574097

The Goat Polyclonal anti-DLGAP1 antibody has been validated for IHC and IHC (p). It is suitable to detect DLGAP1 in samples from Human, Rat, Mouse, Dog, Bat, Cow, Hamster, Horse, Rabbit and Xenopus laevis.

Quick Overview for DLGAP1 antibody (AA 391-405) (ABIN574097)

Target: DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))

Reactivity: Human, Rat, Mouse, Dog, Bat, Cow, Hamster, Horse, Rabbit, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This DLGAP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DLGAP1 Antibody

Binding Specificity: AA 391-405

Specificity: Human DLGAP1. This antibody is expected to recognize both reported isoforms (NP_001003809.1, NP_004737.2)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Xenopus (100%) Turkey, Chicken, Seabass, Pufferfish, Zebrafish (93%) Stickleback (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KISNEHSPKLQIRSH from an internal region of human DLGAP1 (NP_004737.2, NP_001003809.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Xenopus (100%), Turkey, Chicken, Seabass, Pufferfish, Zebrafish (93%), Stickleback (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DLGAP1

Target: DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))

Alternative Name: DLGAP1 / SAPAP1 / GKAP

Background: Name/Gene ID: DLGAP1

Synonyms: DLGAP1, DAP-1, DAP-1-ALPHA, HGKAP, PSD-95/SAP90 binding protein 1, PSD-95/SAP90-binding protein 1, SAPAP1, DAP-1-BETA, DAP1, GKAP

Gene ID: 9229

NCBI Accession: NP_004737, NP_001003809

UniProt: O14490