PRNP antibody

Catalog No. ABIN574438

This Mouse Monoclonal antibody specifically detects PRNP in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PRNP antibody (ABIN574438)

Target: PRNP (Prion Protein (PRNP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EM-20

Product Details anti-PRNP Antibody

Specificity: The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~40 kD, monoglycosylated form ~30 kD, and nonglycosylated form ~19-21 kD. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.

Purification: Protein A purified

Immunogen: Recombinant human prion protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody,

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: PRNP / PrP / Prion

Background: Name/Gene ID: PRNP

Synonyms: PRNP, AltPrP, ASCR, CD230 antigen, CJD, KURU, Major prion protein, p27-30, Prion, Prion-related protein, PRIP, Prion protein (p27-30), PrP, PrP33-35C, BSE, CD230, Prion protein, PrP27-30, PrPc

Gene ID: 5621

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation