CD40 antibody (Extracellular Domain) (PE)

Catalog No. ABIN576317

The Rat Monoclonal anti-CD40 antibody is suitable to detect CD40 in samples from Mouse. It has been validated for FACS, ELISA, IHC and IHC (fro).

Quick Overview for CD40 antibody (Extracellular Domain) (PE) (ABIN576317)

Target: CD40

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD40 antibody is conjugated to PE

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD40 Antibody (PE)

Binding Specificity: Extracellular Domain

Specificity: Recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity ofwas demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40. Is a powerful activator of normal B cells especially in the presence of IL-4.

Purification: Protein G purified

Immunogen: Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.

Type of Immunogen: Fusion protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo (1:1), IHC, IHC-Fr

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: Name/Gene ID: CD40
Family: TNF Receptor

Synonyms: CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5

Gene ID: 958

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints