CD28 antibody (PE)

Catalog No. ABIN576560

The Rat Monoclonal anti-CD28 antibody has been validated for FACS, IHC and IHC (fro). It is suitable to detect CD28 in samples from Human.

Quick Overview for CD28 antibody (PE) (ABIN576560)

Target: CD28

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD28 Antibody (PE)

Specificity: Recognizes the human CD28 cell surface antigen, a 44kD glycoprotein expressed by a subset of T lymphocytes. It has been reported that this Recognizes an epitope of CD28 expressed by NK cells, which is not recognized by clones of 9.3 or CD28.2(1.).

Purification: Protein G purified

Immunogen: Human peripheral blood T-cells.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo (1:1), IHC, IHC-Fr

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL human whole blood. Method sheets are available on request.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: Name/Gene ID: CD28

Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response