MHC Class II (RT1Bu) antibody (PE)
This Mouse Monoclonal antibody specifically detects in FACS. It exhibits reactivity toward Rat and Mouse.
Catalog No. ABIN576931
Quick Overview for MHC Class II (RT1Bu) antibody (PE) (ABIN576931)
Target
MHC Class II (RT1Bu)
Reactivity
Rat, Mouse
Host
Mouse
Clonality
Monoclonal
Conjugate
PE
Application
Flow Cytometry (FACS)
Clone
OX-3
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Specificity
- Recognizes a polymorphic determinant of rat Ia antigen (RT1. Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. Is useful for distinguishing Ia positive cells from different rat strains, e. g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimaeras. MRC OX-3 Reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells. This product is routinely tested in flow cytometry on Lewis rat splenocytes.
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Purification
- Ion exchange chromatography
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Immunogen
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Rat thymocyte membrane glycoproteins.
Type of Immunogen: Membrane extract -
Isotype
- IgG1
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Application Notes
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Approved: Flo (1:1)
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Comment
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Target Species of Antibody: Rat
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Distilled Water.
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Concentration
- Lot specific
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Buffer
- Lyophilized, PBS, pH 7.4, 0.09 % sodium azide, 1 % BSA.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- 4°C, avoid freezing.
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- MHC Class II (RT1Bu)
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Alternative Name
- MHC Class II RT-1Bu
Target
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