Glutathione antibody (DyLight 488)

Catalog No. ABIN577696

This Mouse Monoclonal antibody specifically detects Glutathione in FACS and IF. It exhibits reactivity toward .

Quick Overview for Glutathione antibody (DyLight 488) (ABIN577696)

Target: Glutathione

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glutathione antibody is conjugated to DyLight 488

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: L4H

Product Details anti-Glutathione Antibody (DyLight 488)

Brand: AbX™

Characteristics: Designed for Immunofluorescence and FACS Analysis Supplied as 50 µg in PBS For use under Non-Reducing conditions

Purification: Purified

Immunogen: Glutathione conjugated to Keyhole Limpet Hemocyanin

Isotype: IgG2a

Application Details

Application Notes: 1:5-1:50 IMMuNOFLuOresCeNCe Jurkat cells, permeabilized and incubated with our DyLight® 488 labeled Glutathione monoclo- nal, A001F-100UL, clone L4H, at a 1:25 dilution. 488 Filter unfiltered
Flow analysis of Jurkat cells processed for immunofluorescence as above. Unstained cells Stained at 1x Stained at 5x

Comment:

Designed for Immunofluorescence and FACS Analysis Supplied as 50 μg in PBS For use under Non-Reducing conditions

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 500 μg/mL in Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C

Storage Comment: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Target Details for Glutathione

Target: Glutathione

Target Type: Chemical

Background: Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios. The AbX™ DyLight® 488-labeled dye is similar to Alexa 488, Cy2 or FITC. It will measure GSH-protein complexes under non-reducing conditions.