Glutathione antibody (DyLight 550)

Catalog No. ABIN577697

The Mouse Monoclonal anti-Glutathione antibody has been validated for FACS. It is suitable to detect Glutathione in samples from .

Quick Overview for Glutathione antibody (DyLight 550) (ABIN577697)

Target: Glutathione

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glutathione antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Clone: L4H

Product Details anti-Glutathione Antibody (DyLight 550)

Brand: AbX™

Characteristics: Designed for Immunofluorescence and FACS Analysis
Supplied as 50 µg in PBS
For use under Non-Reducing conditions

Purification: Protein A Purified

Immunogen: Glutathione conjugated to Keyhole Limpet Hemocyanin

Isotype: IgG2a

Application Details

Application Notes: 1:5-1:50 AbX™ DyLight®549- Anti-Glutathione Mouse Monoclonal
Flow analysis of Jurkat cells using DyLight® 549 labeled monoclonal to Glutathione. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Comment:

Designed for Immunofluorescence and FACS Analysis Supplied as 50 μg in PBS For use under Non-Reducing conditions

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 500 μg/mL in Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C

Storage Comment: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Target Details for Glutathione

Target: Glutathione

Target Type: Chemical

Background: Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios. AbX™ DyLight® 549-labeled Glutathione Mouse Monoclonal Antibody is produced from our Protein A purified anti- body by reaction with DyLight® 549 NHS ester. It will fluorescently label GSH-protein complexes under non-reducing conditions. DyLight 549 is similar to Alexa 555, Cy3 or TRITC.