Ubiquitin antibody (Lys63)

Catalog No. ABIN5958536

This Rabbit Polyclonal antibody specifically detects Ubiquitin in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Ubiquitin antibody (Lys63) (ABIN5958536)

Target: Ubiquitin (Ub)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Ubiquitin Antibody

Binding Specificity: Lys63

Specificity: UBB,Polyubiquitin-B,UBC,Polyubiquitin-C,RPS27A,UBA80,UBCEP1,Ubiquitin-40S ribosomal protein S27a,Ubiquitin carboxyl extension protein 80,UBA52,UBCEP2Ubiquitin-60S ribosomal protein L40,CEP52,Ubiquitin A-52 residue ribosomal protein fusion product 1

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human Ub around the non-acetylation site of K63.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA 1:20000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ub

Background: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway