IRF4 antibody

Catalog No. ABIN596032

This Rabbit Monoclonal antibody specifically detects IRF4 in WB and IP. It exhibits reactivity toward Human and Rat.

Quick Overview for IRF4 antibody (ABIN596032)

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IRF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-IRF4 Antibody

Specificity: Recognizes endogenous levels of human total IRF-4 protein. Species cross-reactivity: rat.

Purification: Purified

Immunogen: Synthetic peptide corresponding to residues around Asp175 of human IRF-4 (KLH-coupled).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP (1:100), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000 Incubate membrane with diluted antibody in 5 % w/v BSA, 1X TBS, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight. Immunoprecipitation: 1:100. Chromatin IP: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. For long-term storage and to avoid freeze-thaw cycles, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C.

Target Details for IRF4

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Alternative Name: IRF4 / IRF-4

Background: Name/Gene ID: IRF4

Synonyms: IRF4, Interferon regulatory factor 4, NF-EM5, LSIRF, IRF-4, Multiple myeloma oncogene 1

Gene ID: 3662

UniProt: Q15306

Pathways: Chromatin Binding