Histone H3.3 antibody (Ser31)

Catalog No. ABIN5960674

The Rabbit Polyclonal anti-Histone H3.3 antibody (ABIN5960674) specifically detects Histone H3.3 in WB, ELISA, IF and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Histone H3.3 antibody (Ser31) (ABIN5960674)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H3.3 Antibody

Binding Specificity: Ser31

Specificity: H3 histone family 3A,H3 histone family 3B,H3 histone,family 3B (H3.3B),H3.3,H3.3A,H3.3B,H33,H3F3,H3F3A,H3f3b,Histone H3.3,Histone H3.3Q,Histone H3.A,Histone H3.B,MGC87782,MGC87783

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human Histone H3.3 around the non-phosphorylation site of Ser31.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Molecular Weight: 15kDa

Gene ID: 3020, 3021

UniProt: P84243