Histone H3.3 antibody (Ser31)

Catalog No. ABIN5960674

This anti-Histone H3.3 antibody is a Rabbit Polyclonal antibody detecting Histone H3.3 in ELISA, WB, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Histone H3.3 antibody (Ser31) (ABIN5960674)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H3.3 Antibody

Binding Specificity: Ser31

Specificity: H3 histone family 3A,H3 histone family 3B,H3 histone,family 3B (H3.3B),H3.3,H3.3A,H3.3B,H33,H3F3,H3F3A,H3f3b,Histone H3.3,Histone H3.3Q,Histone H3.A,Histone H3.B,MGC87782,MGC87783

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human Histone H3.3 around the non-phosphorylation site of Ser31.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Molecular Weight: 15kDa

Gene ID: 3020, 3021

UniProt: P84243