Keratin Basic antibody

Catalog No. ABIN596196

This anti- antibody is a Mouse Monoclonal antibody detecting in WB, IHC, IHC (p), IHC (fro) and IP. Suitable for Rat, Mouse, Rabbit, Cow and Chicken.

Quick Overview for Keratin Basic antibody (ABIN596196)

Target: Keratin Basic

Reactivity: Rat, Mouse, Rabbit, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: AE-3

Product Details

Specificity: Recognizes most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. Broadly reactive with almost all epithelial and their neoplasms. Species cross-reactivity: Rabbit, mouse, rat, bovine, chicken.

Purification: Purified

Immunogen: Keratin, Epithelial, Type II.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P, IP, WB (1:100)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100 Detects distinct MW's of 52, 54, 56, 58, 59, 64, and 65-67kD with all Ip's lower then 6.0. These MW's are believed to correspond with keratin 8, 7, 6, 5, 4, 3 and 1 & 2. Immunohistochemistry: 1:150-1:200 for use on formalin-fixed paraffin embedded tissues. Incubate for 30 minutes at 37 °C. Can also be used with unfixed frozen sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Buffer contains: 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details

Target: Keratin Basic

Alternative Name: Basic Cytokeratin