Keratin Basic antibody

Catalog No. ABIN596196

The Mouse Monoclonal anti-Keratin Basic antibody (Clone AE-3) (ABIN596196) specifically detects Keratin Basic in WB, IHC, IHC (p), IHC (fro) and IP. The antibody is reactive with Rat, Mouse, Rabbit, Cow and Chicken samples.

Quick Overview for Keratin Basic antibody (ABIN596196)

Target: Keratin Basic

Reactivity: Rat, Mouse, Rabbit, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: AE-3

Product Details

Specificity: Recognizes most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. Broadly reactive with almost all epithelial and their neoplasms. Species cross-reactivity: Rabbit, mouse, rat, bovine, chicken.

Purification: Purified

Immunogen: Keratin, Epithelial, Type II.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P, IP, WB (1:100)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100 Detects distinct MW's of 52, 54, 56, 58, 59, 64, and 65-67kD with all Ip's lower then 6.0. These MW's are believed to correspond with keratin 8, 7, 6, 5, 4, 3 and 1 & 2. Immunohistochemistry: 1:150-1:200 for use on formalin-fixed paraffin embedded tissues. Incubate for 30 minutes at 37 °C. Can also be used with unfixed frozen sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Buffer contains: 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details

Target: Keratin Basic

Alternative Name: Basic Cytokeratin