5-Methylcytidine antibody

Catalog No. ABIN596516

The Mouse Monoclonal anti-5-Methylcytidine antibody has been validated for FACS, IF, IHC (fro), IHC (p), WB, IHC and IP. It is suitable to detect 5-Methylcytidine in samples from .

Quick Overview for 5-Methylcytidine antibody (ABIN596516)

Target: 5-Methylcytidine

Host: Mouse

Clonality: Monoclonal

Conjugate: This 5-Methylcytidine antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-5-Methylcytidine Antibody

Specificity: Recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. Species cross-reactivity: human, rat, mouse.

Purification: Purified

Immunogen: 5-Methylcytidine.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IF, IHC, IHC-Fr, IHC-P, IP, WB

Usage: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Methylated DNA Immunoprecipitation, and Immunohistochemistry (frozen and paraffin). Flow Cytometry: 10 μL labels 10^6 cells in 100 μL. Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details for 5-Methylcytidine

Target: 5-Methylcytidine

Target Type: Chemical