PRSS3 antibody

Catalog No. ABIN598236

This anti-PRSS3 antibody is a Rat Monoclonal antibody detecting PRSS3 in WB and Neut. Suitable for Human.

Quick Overview for PRSS3 antibody (ABIN598236)

Target: PRSS3 (Protease, serine, 3 (PRSS3))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This PRSS3 antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut)

Product Details anti-PRSS3 Antibody

Specificity: Recognizes both pro and mature forms of human trypsin 3. Species sequence homology: chimpanzee: 96 % , rat: 76 %, mouse: 74 %, dog: 72 %. and chicken: 67 %. Does not cross-react with rhTrypsin-1 or rhTrypsin-2.

Purification: Protein G purified

Immunogen: NS0-derived recombinant human rhTrypsin-3.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in Neutralization and Western Blot. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50 % of the proteolytic activity was inhibited by the antibody at approximately 0.15 μg/mL (IC 50) under conditions in which the enzyme was present at 0.03 μg/mL and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 Molar ratio of the antibody to the enzyme. Western blot:1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhTrypsin-3 is ~25 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Reducing conditions are not recommended.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile buffer.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for PRSS3

Target: PRSS3 (Protease, serine, 3 (PRSS3))

Alternative Name: PRSS3 / Trypsin 3

Background: Name/Gene ID: PRSS3
Subfamily: Serine S1
Family: Protease

Synonyms: PRSS3, Brain trypsinogen, Mesotrypsin, PRSS4, Serine protease 4, Serine protease 3, TRY4, Trypsin III, Trypsin IV, Trypsinogen 4, MTG, Trypsinogen 5, Pancreatic trypsinogen III, Protease, serine, 3, Trypsin-3, Mesotrypsinogen, T9, TRY3, Trypsinogen IV

Gene ID: 5646

UniProt: P35030