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NEFH antibody (C-Term)

This Mouse Monoclonal antibody specifically detects NEFH in WB, IHC, IHC (p), IF, IHC (fro) and IP. It exhibits reactivity toward Human, Rat and Mouse.
Catalog No. ABIN599659

Quick Overview for NEFH antibody (C-Term) (ABIN599659)

Target

See all NEFH Antibodies
NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity

  • 127
  • 96
  • 75
  • 60
  • 41
  • 33
  • 24
  • 22
  • 13
  • 13
  • 9
  • 9
  • 9
  • 7
  • 7
  • 5
  • 2
  • 2
  • 1
  • 1
Human, Rat, Mouse

Host

  • 80
  • 63
  • 9
Mouse

Clonality

  • 98
  • 54
Monoclonal

Conjugate

  • 95
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NEFH antibody is un-conjugated

Application

  • 100
  • 60
  • 48
  • 31
  • 26
  • 23
  • 20
  • 18
  • 15
  • 14
  • 13
  • 13
  • 11
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
  • Binding Specificity

    • 16
    • 10
    • 7
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificity

    Recognizes NF200. Species cross-reactivity: human, mouse, rat.

    Purification

    Immunoaffinity purified

    Immunogen

    C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

    Type of Immunogen: Fusion protein

    Isotype

    IgG1
  • Application Notes

    Approved: IF, IHC, IHC-Fr (1 - 2 μg/mL), IHC-P (1 - 2 μg/mL), IP, WB (1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunofluorescence. Western Blot: 0.5ml. Immunohistochemistry (paraffin, frozen): 1-2 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    1.2 % sodium acetate

    Concentration

    Lot specific

    Buffer

    Lyophilized from 1.2 % sodium acetate, 2 mg BSA, 0.01 mg sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Aliquot to Avoid repeated freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
  • Target

    NEFH (Neurofilament, Heavy Polypeptide (NEFH))

    Alternative Name

    NEFH / NF-H

    Background

    Name/Gene ID: NEFH

    Synonyms: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

    Gene ID

    4744

    UniProt

    P12036
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