Sonic Hedgehog antibody (N-Term)

Catalog No. ABIN600206

The Rabbit Polyclonal anti-Sonic Hedgehog antibody has been validated for WB, IHC, ICC and IHC (p). It is suitable to detect Sonic Hedgehog in samples from Human, Mouse and Rat.

Quick Overview for Sonic Hedgehog antibody (N-Term) (ABIN600206)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: N-Term

Specificity: Recognizes human SHH. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to a sequence near the N-terminal of human SHH, identical to the related mouse and rat sequence.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (1 - 2 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot:1-2 μg/mL. Immunohistochemistry (paraffin):1-2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % BSA, 0.05 % thimerosal, 0.05 % sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog / SHH

Background: Name/Gene ID: SHH
Subfamily: Cysteine C46
Family: Protease

Synonyms: SHH, HHG-1, HLP3, Sonic hedgehog, Sonic hedgehog homolog, MCOPCB5, Sonic hedgehog protein, HHG1, HPE3, SMMCI, TPT, TPTPS

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development