SPARC antibody

Catalog No. ABIN602083

This Rat Monoclonal antibody specifically detects SPARC in IHC, ELISA, FACS and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for SPARC antibody (ABIN602083)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SPARC Antibody

Specificity: Recognizes mouse Osteonectin. Displays no cross-reactivity with recombinant human Osteonectin.

Purification: Protein G purified

Immunogen: Recombinant mouse Osteonectin.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-Fr (25 μg/mL)

Usage: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. ELISA: 0.5-1 μg/mL). Immunohistochemistry (frozen): 25 μg/mL detects SPARC on mouse ovary tissue. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 25 μg/mL and add 10 μLof this solution to 1-5x10^5 cells in a total reaction volume 200uL. Following a 30 minute incubation, cells should be washed with 0.1 % saponin prior to adding 10 mL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C. Further dilutions can be made in buffer.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: Osteonectin / SPARC

Background: Name/Gene ID: SPARC

Synonyms: SPARC, Basement-membrane protein 40, BM-40, Cysteine-rich protein, ON, Osteonectin

Gene ID: 6678

UniProt: P09486

Pathways: Autophagy