SPARC antibody
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- Target See all SPARC Antibodies
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This SPARC antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Recognizes mouse Osteonectin. Displays no cross-reactivity with recombinant human Osteonectin.
- Purification
- Protein G purified
- Immunogen
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Recombinant mouse Osteonectin.
Type of Immunogen: Recombinant protein - Isotype
- IgG2b
- Top Product
- Discover our top product SPARC Primary Antibody
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anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 101-200) antibody
SPARC Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1B2 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251) antibodySPARC Reactivity: Human WB, IHC, ELISA, FACS Host: Rabbit Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibodySPARC Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB19869 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303) antibodySPARC Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) antibodySPARC Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303), (C-Term) antibodySPARC Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibodySPARC Reactivity: Human WB Host: Mouse Monoclonal 664CT7-3-1 unconjugated
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- Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-Fr (25 μg/mL)
Usage: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. ELISA: 0.5-1 μg/mL). Immunohistochemistry (frozen): 25 μg/mL detects SPARC on mouse ovary tissue. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 25 μg/mL and add 10 μLof this solution to 1-5x10^5 cells in a total reaction volume 200uL. Following a 30 minute incubation, cells should be washed with 0.1 % saponin prior to adding 10 mL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile PBS
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.4, 5 % trehalose
- Storage
- -20 °C
- Storage Comment
- Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C. Further dilutions can be made in buffer.
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- Target
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
- Alternative Name
- Osteonectin / SPARC (SPARC Products)
- Background
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Name/Gene ID: SPARC
Synonyms: SPARC, Basement-membrane protein 40, BM-40, Cysteine-rich protein, ON, Osteonectin - Gene ID
- 6678
- UniProt
- P09486
- Pathways
- Autophagy
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