Cytotoxic Necrotizing Factor 1 & 2 antibody
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- Target
- Cytotoxic Necrotizing Factor 1 & 2
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Reactivity
- E. coli
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Functional Studies (Func)
- Specificity
- Specific for Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic Escherichia coli. Recognizes an epitope between aa169-191 of the N-terminal binding domain. It neutralizes only CNF1.
- Purification
- Purified
- Immunogen
- E. coli CNF1/CNF2.
- Isotype
- IgG2a
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- Application Notes
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Approved: Func, WB (1:10)
Usage: Suitable for use in Immunoassays, Western Blot, and inhibition of toxin activity of CNF1. JC4 does not inhibit the toxin activity of CNF2. Western Blot: 1:10. - Comment
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Target Species of Antibody: E. coli
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.1 % BSA
- Handling Advice
- Avoid repeated freezing and thawing
- Storage
- 4 °C,-20 °C
- Storage Comment
- May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
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- Target
- Cytotoxic Necrotizing Factor 1 & 2
- Background
- This antibody detects: Cytotoxic Necrotizing Factor 1+2
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