LGALS14 antibody (AA 1-139)

Catalog No. ABIN603241

This Mouse Monoclonal antibody specifically detects LGALS14 in ELISA and WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for LGALS14 antibody (AA 1-139) (ABIN603241)

Target: LGALS14 (Lectin, Galactoside-Binding, Soluble, 14 (LGALS14))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGALS14 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-LGALS14 Antibody

Binding Specificity: AA 1-139

Specificity: Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9.

Purification: Immunoaffinity purified

Sterility: Sterile filtered

Immunogen: Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14, aa 1-139, Accession# Q8TCE9).

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (2 μg/mL)

Usage: Suitable for use in Western blot: 2 μg/mL with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10 μg total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25 mM Tris,  pH 7.4 5 % nonfat dry milk 5 % nonfat dry milk 0.15 M NaCl in Blotting Buffer in Blotting Buffer 0.05 % Tween 20. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from sterile-filtered PBS, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.

Target Details for LGALS14

Target: LGALS14 (Lectin, Galactoside-Binding, Soluble, 14 (LGALS14))

Alternative Name: LGALS14 / CLC2

Background: Name/Gene ID: LGALS14

Synonyms: LGALS14, CLC2, Gal-14, Galectin-14, Placental protein 13-like, PPL13

Gene ID: 56891

UniProt: Q8TCE9