PDHA1 antibody (pSer293)

Catalog No. ABIN603718

This Rabbit Polyclonal antibody specifically detects PDHA1 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for PDHA1 antibody (pSer293) (ABIN603718)

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PDHA1 Antibody

Binding Specificity: pSer293

Specificity: Recognizes human Pyruvate Dehydrogenase E1-alpha Subunit when phosphorylated at Serine 293.

Purification: Purified

Immunogen: Synthetic peptide corresponding to surrounding the phosphorylated serine 293 of the human PDHE1 alpha protein. [Swiss-Prot #P08559].

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:50 - 1:200), IF (1:50 - 1:200), WB (1:1000 - 1:5000)

Usage: Suitable for use in Immunocytochemistry, Immunofluorescence and Suitable for use in Western blot: 1:1000-1:5000 detects a band at ~43kD. Immunocytochemistry: 1:50-1:200. Immunofluorescence: 1:50-1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate,  pH 7.5, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for PDHA1

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Alternative Name: PDHA1 / PDH E1 alpha

Background: Name/Gene ID: PDHA1

Synonyms: PDHA1, PDHCE1A, PDHA, PHE1A, PDHE1-A type I

Gene ID: 5160

UniProt: P08559

Pathways: Warburg Effect