Transferrin antibody (AA 20-698)

Catalog No. ABIN603882

This anti-Transferrin antibody is a Mouse Monoclonal antibody detecting Transferrin in WB and ELISA. Suitable for Human.

Quick Overview for Transferrin antibody (AA 20-698) (ABIN603882)

Target: Transferrin (TF)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Transferrin Antibody

Binding Specificity: AA 20-698

Specificity: Detects rhTransferrin in direct ELISAs and Western blots.

Purification: Immunoaffinity purified

Sterility: Sterile filtered

Immunogen: Purified, Sfr-derived, recombinant human Transferrin (rhTransferrin, aa 20-698, Accession# P02787).

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (2 μg/mL)

Usage: Suitable for use in ELISA, Suitable for use in Western blot: 2 μg/mL with the appropriate secondary reagents to detect human Transferrin in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from human liver extract in non-reducing sample buffer, resolved by SDS-PAGE (30 μg total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2 μg/mL and. chemiluminescent detection substrate. Use of this antibody under reducing condition is not. recommended. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human Transferrin. The detection limit for rhTransferrin is approximately 1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from sterile-filtered PBS, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: TF / Transferrin

Background: Name/Gene ID: TF

Synonyms: TF, PRO1557, Serotransferrin, Siderophilin, TFQTL1, Beta-1 metal-binding globulin, Transferrin, PRO2086

Gene ID: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis