Histone H4 antibody (2meLys20)

Catalog No. ABIN605115

This anti-Histone H4 antibody is a Mouse Monoclonal antibody detecting Histone H4 in WB, IHC, IP and IHC (p). Suitable for Human, Mouse, Rat and Cow.

Quick Overview for Histone H4 antibody (2meLys20) (ABIN605115)

Target: Histone H4

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H4 Antibody

Binding Specificity: 2meLys20, AA 17-28

Brand: IHC-plus™

Specificity: Recognizes methyl-Histone H4, Mr 10kD. Species cross-reactivity: Human, mouse, rat, and bovine. Broad species Cross-reactivity predicted based on conservation of sequence. Does not cross-react with yeast or Tetrahymena.

Purification: Protein G purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 (RHR[dimeK] VLRDNIQGC) of human histone H4.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB (0.01 - 0.1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 0.01-0.1 μg/mL detects methyl-histone H4 in HeLa acid extracts. Minimal cross-reactivity with unmethylated recombinant Histone H4 is observed if used at these concentrations. Immunohistochemistry: stains Drosophila polytene chromosomes as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates methyl-Histone H4 from HeLa cell lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. Sourced in Ascites.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Histone H4

Target: Histone H4