Histone H4 antibody (2meLys20)

Catalog No. ABIN605115

The Mouse Monoclonal anti-Histone H4 antibody (ABIN605115) specifically detects Histone H4 in WB, IHC, IP and IHC (p). The antibody is reactive with Human, Mouse, Rat and Cow samples.

Quick Overview for Histone H4 antibody (2meLys20) (ABIN605115)

Target: Histone H4

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H4 Antibody

Binding Specificity: 2meLys20, AA 17-28

Specificity: Recognizes methyl-Histone H4, Mr 10kD. Species cross-reactivity: Human, mouse, rat, and bovine. Broad species Cross-reactivity predicted based on conservation of sequence. Does not cross-react with yeast or Tetrahymena.

Purification: Protein G purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 (RHR[dimeK] VLRDNIQGC) of human histone H4.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB (0.01 - 0.1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 0.01-0.1 μg/mL detects methyl-histone H4 in HeLa acid extracts. Minimal cross-reactivity with unmethylated recombinant Histone H4 is observed if used at these concentrations. Immunohistochemistry: stains Drosophila polytene chromosomes as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates methyl-Histone H4 from HeLa cell lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. Sourced in Ascites.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Histone H4

Target: Histone H4