CD28 antibody (FITC)

Catalog No. ABIN610014

This anti-CD28 antibody is a Mouse Monoclonal antibody detecting CD28 in FACS, ELISA and Func. Suitable for Human.

Quick Overview for CD28 antibody (FITC) (ABIN610014)

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), ELISA, Functional Studies (Func)

Clone: ANC28-1-5D10

Product Details anti-CD28 Antibody (FITC)

Specificity: CD28 expressed on T cells and also on BHK cells transfected with human CD28. This antibody has been characterized as a superagonist.

Purification: Protein A purified

Immunogen: Recombinant Human CD28 and HPB-MLT human tumor cells.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo, Func

Usage: Function: ANC28.1 stimulates expression of IL-2 from CD28+ cells. Divalent ANC28.1 can induce Antigen Independent T cell proliferation. In combination with anti-CD81 mAb, it can prime naive T cells to acquire effector function independent of TCR ligation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 5 % Glycerol, 0.2 % BSA, 0.04 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Product is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: Name/Gene ID: CD28

Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response