LTBR antibody (FITC)

Catalog No. ABIN610269

Cited in 3+ publications. The FITC-conjugated Mouse Monoclonal anti-LTBR antibody (Clone ANCLTR2-9E2) (ABIN610269) specifically detects LTBR in ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for LTBR antibody (FITC) (ABIN610269)

Target: LTBR (Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LTBR antibody is conjugated to FITC

Application: ELISA, Flow Cytometry (FACS)

Clone: ANCLTR2-9E2

Product Details anti-LTBR Antibody (FITC)

Specificity: Human LTbR present on U-937 cell surface, and to recombinant LTbR-muIg in EIA.

Purification: Protein A purified

Immunogen: Recombinant Human LT.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Five x 10e5 cultured human U-937 cells were pre incubated with 20 μL of 150 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of antibody LS-C134619 at a 1:50 dilution (10 ?g/mL). They were then washed three times, fixed and analyzed by FACS.Or the antibody can be used at 1.6 μL of concentrated stock per 5 x 10e5-cell test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 5 % Glycerol, 0.2 % BSA, 0.04 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Product is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

References for anti-LTBR antibody (FITC) (ABIN610269)

Seki, Takasu, Mandai, Nakata, Saeki, Heike, Takata, Segawa, Hanafusa, Eguchi: "Expression of eukaryotic initiation factor 4E in atypical adenomatous hyperplasia and adenocarcinoma of the human peripheral lung." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 8, Issue 10, pp. 3046-53, (2002) (PubMed).

Jiang, Ballou, Lin: "Rapamycin-insensitive regulation of 4e-BP1 in regenerating rat liver." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 10943-51, (2001) (PubMed).

De Benedetti, Rhoads: "Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrant growth and morphology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 21, pp. 8212-6, (1990) (PubMed).

Target Details for LTBR

Target: LTBR (Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))

Alternative Name: LTBR

Background: Name/Gene ID: LTBR
Family: TNF Receptor

Synonyms: LTBR, D12S370, CD18, LT-BETA-R, Lymphotoxin B receptor, Lymphotoxin-beta receptor, TNFR3, TNF-R-III, TNF-RIII, TNFCR, TNFRSF3, TNFR-III, TNFR-RP, TNFR2-RP

Gene ID: 4055

UniProt: P36941

Pathways: NF-kappaB Signaling