Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 594)

Catalog No. ABIN612377

Quick Overview for Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 594) (ABIN612377)

Target: IgG

Conjugate: DyLight 594

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Specificity: Based on IEP, this antibody reacts with:
heavy (γ) chains on rabbit IgG
light chains on all rabbit immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: non-immunoglobulin rabbit serum proteins

Characteristics: Goat anti-rabbit IgG (H&L) - Affinity Pure, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.

Purification: Affinity purified using solid phase Rabbit IgG

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified rabbit IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C/-20 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.
Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.

Target Details

Target: IgG

Target Type: Antibody