CDT2/RAMP antibody (AA 541-730)

Catalog No. ABIN6139847

The Rabbit Polyclonal anti-CDT2/RAMP antibody has been validated for WB. It is suitable to detect CDT2/RAMP in samples from Human.

Quick Overview for CDT2/RAMP antibody (AA 541-730) (ABIN6139847)

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDT2/RAMP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDT2/RAMP Antibody

Binding Specificity: AA 541-730

Sequence: AEACSESRNR VKRRLDSSCL ESVKQKCVKS CNCVTELDGQ VENLHLDLCC LAGNQEDLSK DSLGPTKSSK IEGAGTSISE PPSPISPYAS ESCGTLPLPL RPCGEGSEMV GKENSSPENK NWLLAMAAKR KAENPSPRSP SSQTPNSRRQ SGKTLPSPVT ITPSSMRKIC TYFHRKSQED FCGPEHSTEL

Cross-Reactivity: Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 541-730 of human DTL (NP_057532.3).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for CDT2/RAMP

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Alternative Name: DTL

Background: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL complex, also named CRL4(CDT2 complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1, FBH1, KMT5A and SDE2. CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1 degradation during S phase or following UV irradiation is essential to control replication licensing. KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1.,DTL,CDT2,DCAF2,L2DTL,RAMP,Cell Biology & Developmental Biology,Cell Cycle,Ubiquitin,DTL

Molecular Weight: 23 kDa/79 kDa

Gene ID: 51514

UniProt: Q9NZJ0