MELK antibody (AA 412-651)

Catalog No. ABIN6143725

This anti-MELK antibody is a Rabbit Polyclonal antibody detecting MELK in WB and IHC. Suitable for Human.

Quick Overview for MELK antibody (AA 412-651) (ABIN6143725)

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MELK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MELK Antibody

Binding Specificity: AA 412-651

Sequence: LCRTPANKLK NKENVYTPKS AVKNEEYFMF PEPKTPVNKN QHKREILTTP NRYTTPSKAR NQCLKETPIK IPVNSTGTDK LMTGVISPER RCRSVELDLN QAHMEETPKR KGAKVFGSLE RGLDKVITVL TRSKRKGSAR DGPRRLKLHY NVTTTRLVNP DQLLNEIMSI LPKKHVDFVQ KGYTLKCQTQ SDFGKVTMQF ELEVCQLQKP DVVGIRRQRL KGDAWVYKRL VEDILSSCKV

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 412-651 of human MELK (NP_055606.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:100 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: MELK

Background: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity, phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.,MELK,HPK38,Signal Transduction,Kinase,Cell Biology & Developmental Biology,Cell Cycle,Stem Cells,Embryonic Stem Cells,MELK

Molecular Weight: 52 kDa/59 kDa/66 kDa/69 kDa/70 kDa/71 kDa/74 kDa

Gene ID: 9833

UniProt: Q14680