NEDD4-2 antibody (AA 836-955)

Catalog No. ABIN6144504

The Rabbit Polyclonal anti-NEDD4-2 antibody has been validated for WB, IHC and IF. It is suitable to detect NEDD4-2 in samples from Human.

Quick Overview for NEDD4-2 antibody (AA 836-955) (ABIN6144504)

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD4-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NEDD4-2 Antibody

Binding Specificity: AA 836-955

Sequence: GDVDVNDWRQ HSIYKNGYCP NHPVIQWFWK AVLLMDAEKR IRLLQFVTGT SRVPMNGFAE LYGSNGPQLF TIEQWGSPEK LPRAHTCFNR LDLPPYETFE DLREKLLMAV ENAQGFEGVD

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Alternative Name: NEDD4L

Background: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.,NEDD4L,NEDD4-2,NEDD4.2,PVNH7,RSP5,hNEDD4-2,Epigenetics & Nuclear Signaling,Cell Biology & Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,NEDD4L

Molecular Weight: 96-111 kDa

Gene ID: 23327

UniProt: Q96PU5

Pathways: Negative Regulation of Transporter Activity