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NEDD4-2 antibody (Middle Region)

NEDD4L Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2774731
  • Target See all NEDD4-2 (NEDD4L) Antibodies
    NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))
    Binding Specificity
    • 15
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    • 66
    • 43
    • 15
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)
    Host
    • 75
    • 4
    Rabbit
    Clonality
    • 69
    • 10
    Polyclonal
    Conjugate
    • 30
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NEDD4-2 antibody is un-conjugated
    Application
    • 36
    • 25
    • 13
    • 13
    • 7
    • 7
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    TVTLSAPLEG AKDSPVRRAV KDTLSNPQSP QPSPYNSPKP QHKVTQSFLP
    Predicted Reactivity
    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
    Characteristics
    This is a rabbit polyclonal antibody against NEDD4L. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L
    Top Product
    Discover our top product NEDD4L Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 955 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Raikwar, Thomas: "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1157-65, (2008) (PubMed).

  • Target
    NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))
    Alternative Name
    NEDD4L (NEDD4L Products)
    Synonyms
    NEDD4-2 antibody, NEDD4.2 antibody, RSP5 antibody, hNEDD4-2 antibody, nedd4 antibody, nedd4-2 antibody, NEDD4 antibody, Nedd4-2 antibody, 1300012C07Rik antibody, Nedd4b antibody, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase antibody, NEDD4L antibody, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase S homeolog antibody, neural precursor cell expressed, developmentally down-regulated gene 4-like antibody, NEDD4L antibody, LOC776799 antibody, nedd4l.S antibody, Nedd4l antibody
    Background
    NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
    Alias Symbols: FLJ33870, KIAA0439, RSP5, hNedd4-2, NEDD4-2, NEDD4.2, hNEDD4-2
    Protein Interaction Partner: NEDD4L, DAZAP2, UBE2D1, PTCH1, WNK3, TNK2, SNCA, SLC9A3, SMAD6, KCNH2, HCN1, SMAD3, UBE2N, SNA3, NOTCH1, UBE2D3, UBC, SGK1, KCNQ1, SGK3, GORAB, MED28, FBXL15, vpr, RNF11, AATF, YWHAQ, LAPTM5, SCNN1B, SMAD2, ARRDC3, ARRDC4, UBE2L3, UBE2D2, SMAD7, UBE2K, UB
    Protein Size: 955
    Molecular Weight
    110 kDa
    Gene ID
    23327
    NCBI Accession
    NM_015277, NP_056092
    Pathways
    Negative Regulation of Transporter Activity
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