RFWD2 antibody (AA 582-731)

Catalog No. ABIN6146897

This Rabbit Polyclonal antibody specifically detects RFWD2 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for RFWD2 antibody (AA 582-731) (ABIN6146897)

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFWD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RFWD2 Antibody

Binding Specificity: AA 582-731

Sequence: YYDLRNTKQP IMVFKGHRKA VSYAKFVSGE EIVSASTDSQ LKLWNVGKPY CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human RFWD2 (NP_071902.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:100 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for RFWD2

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Alternative Name: RFWD2

Background: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53 ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable.,RFWD2,COP1,RNF200,Epigenetics & Nuclear Signaling,Cell Biology & Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,RFWD2

Molecular Weight: 14 kDa/57 kDa/77 kDa/78 kDa/80 kDa

Gene ID: 64326

UniProt: Q8NHY2

Pathways: Photoperiodism