USP13 antibody (AA 90-200)

Catalog No. ABIN6149996

This Rabbit Polyclonal antibody specifically detects USP13 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for USP13 antibody (AA 90-200) (ABIN6149996)

Target: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-USP13 Antibody

Binding Specificity: AA 90-200

Sequence: MHLKRHVREK VRGASGGALP KRRNSKIFLD LDTDDDLNSD DYEYEDEAKL VIFPDHYEIA LPNIEELPAL VTIACDAVLS SKSPYRKQDP DTWENELPVS KYANNLTQLD N

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-200 of human USP13 (NP_003931.2).

Isotype: IgG

Application Details

Application Notes: WB,1:1000 - 1:2000,IHC,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for USP13

Target: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Alternative Name: USP13

Background: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD. Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro, however, additional experiments are required to confirm such data.,USP13,ISOT3,IsoT-3,Epigenetics & Nuclear Signaling,Cell Biology & Developmental Biology,Ubiquitin,USP13

Molecular Weight: 90 kDa/97 kDa

Gene ID: 8975

UniProt: Q92995

Pathways: SARS-CoV-2 Protein Interactome