Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HLA-DP beta antibody

This Mouse Monoclonal antibody specifically detects HLA-DP beta in FACS, IF and IHC (f). It exhibits reactivity toward Human.
Catalog No. ABIN6153336

Quick Overview for HLA-DP beta antibody (ABIN6153336)

Target

HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))

Reactivity

Human

Host

  • 17
Mouse

Clonality

  • 17
Monoclonal

Conjugate

  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This HLA-DP beta antibody is un-conjugated

Application

Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

BRA-FB6
  • Purpose

    Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (BRA-FB6), Purified, with BSA

    Characteristics

    This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked i± (33-35 kDa) and i² (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Purification

    Purified

    Immunogen

    Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

    Isotype

    IgG2b kappa
  • Application Notes

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    Comment

    Raji cells. Tonsil or lymph node

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    200 μg/mL

    Buffer

    PBS/0.05 % BSA/0.05 % azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target

    HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))

    Alternative Name

    MHC-II (HLA-DP) (beta chain)

    Molecular Weight

    33-35 kDa

    Gene ID

    3115, 347270

    UniProt

    P04440
You are here:
Chat with us!