HLA-DP beta antibody
Quick Overview for HLA-DP beta antibody (ABIN6153336)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (BRA-FB6), Purified, with BSA
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Characteristics
- This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked i± (33-35 kDa) and i² (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Purification
- Purified
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Immunogen
- Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
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Isotype
- IgG2b kappa
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Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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Comment
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Raji cells. Tonsil or lymph node
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 200 μg/mL
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Buffer
- PBS/0.05 % BSA/0.05 % azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
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Alternative Name
- MHC-II (HLA-DP) (beta chain)
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Molecular Weight
- 33-35 kDa
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Gene ID
- 3115, 347270
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UniProt
- P04440
Target
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