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HLA-DP beta antibody (CF®488A)

HLA-DP beta Reactivity: Human FACS, IF, IHC (f) Host: Mouse Monoclonal Bra-14 CF®488A
Catalog No. ABIN6153361
  • Target See all HLA-DP beta products
    HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
    Reactivity
    Human
    Host
    • 17
    Mouse
    Clonality
    • 17
    Monoclonal
    Conjugate
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This HLA-DP beta antibody is conjugated to CF®488A
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (Bra-14), CF488A Conjugate
    Characteristics
    This antibody recognizes a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Human REH cells
    Clone
    Bra-14
    Isotype
    IgG3 kappa
  • Application Notes
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Raji cells. Tonsil or lymph node

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
    Alternative Name
    MHC-II (HLA-DP) (beta chain) (HLA-DP beta Products)
    Molecular Weight
    36 kDa (? chain) and 27 kDa (? chain)
    Gene ID
    3115, 3122
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