Recombinant WT1 antibody (CF®640R)

Catalog No. ABIN6154673

This anti-WT1 antibody is a Mouse Monoclonal antibody detecting WT1 in IHC, IF and FACS. Suitable for Human.

Quick Overview for Recombinant WT1 antibody (CF®640R) (ABIN6154673)

Target: WT1 (Wilms Tumor 1 (WT1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WT1 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RWT1-857

Product Details anti-WT1 Antibody (CF®640R)

Purpose: Recombinant Mouse Monoclonal anti-Wilm's Tumor 1 (rWT1/857), CF640R Conjugate

Characteristics: This antibody recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human WT1 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

K562 cells. Wilm s Tumor, mesothelioma or fetal kidney.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilm's Tumor 1

Background: WT1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1

Molecular Weight: 47-55 kDa

Gene ID: 7490, 591980

UniProt: P19544

Pathways: Tube Formation