MHC, Class I antibody

Catalog No. ABIN6155390

The Mouse Monoclonal anti-MHC, Class I antibody (Clone 246-B8-E7) (ABIN6155390) specifically detects MHC, Class I in FACS and IF. The antibody is reactive with Mouse, Human and Non-Human Primate samples.

Quick Overview for MHC, Class I antibody (ABIN6155390)

Target: MHC, Class I

Reactivity: Mouse, Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC, Class I antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 246-B8-E7

Product Details anti-MHC, Class I Antibody

Purpose: Mouse Monoclonal anti-MHC-I (246-B8.E7), Purified, BSA-free

Characteristics: This antibody recognizes a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Human peripheral blood leukocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

THP-1 and Jurkat cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: MHC-I

Background: HLA-A, HLA-B, HLA-C, HLA class 1A, HLA class 1B, HLA class 1C, Major histocompatibility complex, class I, A B C, MHC class I HLA-A, MHC class I HLA-B, MHC class I HLA-C, MHC HLA-ABC

Molecular Weight: ~41 kDa

Gene ID: 3105, 181244, 654404

UniProt: P01889, P30443, P30499