PLAP antibody (CF®647)

Catalog No. ABIN6155931

This Mouse Monoclonal antibody specifically detects PLAP in FACS, IF and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PLAP antibody (CF®647) (ABIN6155931)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAP antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ALPP-238

Product Details anti-PLAP Antibody (CF®647)

Purpose: Mouse Monoclonal anti-PLAP (Placental Alkaline Phosphatase) (ALPP/238), CF647 Conjugate

Characteristics: PLAP is a tissue specific, trophoblast-derived, 70 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2 metallo glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP as to AP from normal human testis. This MAb can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human ALPP protein

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology frozen 1-2 μg/mL for 30 minutes at RT

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 cells. Placenta or seminoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: PLAP (Placental Alkaline Phosphatase)

Molecular Weight: 70 kDa

Gene ID: 250, 284255

UniProt: P05187