PLAP antibody (CF®640R)

Catalog No. ABIN6155965

The Mouse Monoclonal anti-PLAP antibody has been validated for IHC, FACS, IF, ISt and IHC (f). It is suitable to detect PLAP in samples from Human.

Quick Overview for PLAP antibody (CF®640R) (ABIN6155965)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAP antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ALPP-870

Product Details anti-PLAP Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-PLAP (Placental Alkaline Phosphatase) (ALPP/870), CF640R Conjugate

Characteristics: This antibody recognizes a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP This antibody recognizes germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human ALPP protein

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 cells. Placenta or seminoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: PLAP (Placental Alkaline Phosphatase)

Molecular Weight: 70 kDa

Gene ID: 250, 284255

UniProt: P05187