F13A1 antibody (AA 46-181) (CF®647)

Catalog No. ABIN6156349

The Mouse Monoclonal anti-F13A1 antibody has been validated for WB, ELISA, IHC, IF, FACS and Coat. It is suitable to detect F13A1 in samples from Human.

Quick Overview for F13A1 antibody (AA 46-181) (CF®647) (ABIN6156349)

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F13A1 antibody is conjugated to CF®647

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Clone: F13A1-1448

Product Details anti-F13A1 Antibody (CF®647)

Binding Specificity: AA 46-181

Purpose: Mouse Monoclonal anti Factor-XIIIa (F13A1/1448), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 83 kDa, which is identified as Factor XIIIa. It has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/ ) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T Cells. Placenta.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Alternative Name: Factor-XIIIa

Molecular Weight: 83 kDa

Gene ID: 2162, 335513

UniProt: P00488