CD2 antibody (CF®647)

Catalog No. ABIN6158135

This anti-CD2 antibody is a Mouse Monoclonal antibody detecting CD2 in FACS and IF. Suitable for Human, Pig and Non-Human Primate.

Quick Overview for CD2 antibody (CF®647) (ABIN6158135)

Target: CD2

Reactivity: Human, Pig, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: LFA2-600

Product Details anti-CD2 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-CD2 (LFA2/600), CF647 Conjugate

Characteristics: CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human LFA2 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T or Jurkat cells. Lymph node and tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD2

Target: CD2

Alternative Name: CD2

Background: Erythrocyte receptor, LFA-2, LFA-3 receptor, Ly-37, Lymphocyte function antigen 2, Rosette receptor, SRBC, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5, T11

Molecular Weight: ~50 kDa

Gene ID: 914, 523500

UniProt: P06729