Recombinant PD-1 antibody (CF®405S)

Catalog No. ABIN6158470

This Rabbit Monoclonal antibody specifically detects PD-1 in FACS, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Recombinant PD-1 antibody (CF®405S) (ABIN6158470)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PD-1 antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: PDCD1-1410R

Product Details anti-PD-1 Antibody (CF®405S)

Purpose: Recombinant Rabbit Monoclonal anti-PDCD1 / PD1 / CD279 (PDCD1/1410R), CF405S Conjugate

Characteristics: PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human recombinant PDCD1 protein fragment (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

TY cells or Tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PDCD1 / PD1 / CD279

Background: CD279, hPD-1, hSLE1, PD1, PDCD1, Programmed Cell Death Protein 1, Protein PD-1, SLEB2, Systemic lupus erythematosus susceptibility 2

Molecular Weight: 55 kDa

Gene ID: 5133, 158297

UniProt: Q15116

Pathways: Cancer Immune Checkpoints