EpCAM antibody (N-Term) (Biotin)

Catalog No. ABIN6159068

This anti-EpCAM antibody is a Mouse Monoclonal antibody detecting EpCAM in WB, FACS, IF and IHC (f). Suitable for Human.

Quick Overview for EpCAM antibody (N-Term) (Biotin) (ABIN6159068)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: EGP40-826

Product Details anti-EpCAM Antibody (Biotin)

Binding Specificity: AA 20-60, N-Term

Purpose: Mouse Monoclonal anti-Ep-CAM / CD326 (EGP40/826), Biotin Conjugate

Characteristics: This antibody recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Does not react with mouse or rat, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HT29 cells or breast tumor

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: Ep-CAM / CD326

Background: Adenocarcinoma-associated Antigen| Cell Surface Glycoprotein Trop-1| EGP2| EGP314| EGP40| Epithelial Cell Adhesion Molecule| Epithelial Glycoprotein 314| ESA| KSA| TACD1| TROP1| Tumor-associated Calcium Signal Transducer 1 (TACSTD1)| ECS-1| Epidermal Surface Antigen 1| ESA1| FLOT2| Flotillin-2| Membrane Component| Chromosome 17| Surface Marker-1 (M17S1)| REG-1| Reggie-1| Reggie-2

Molecular Weight: 40-43 kDa

Gene ID: 4072, 542050

UniProt: P16422