HSPG antibody (Biotin)

Catalog No. ABIN6159600

This Rat Monoclonal antibody specifically detects HSPG in IHC (p), IF, FACS and IHC (f). It exhibits reactivity toward Human, Cow, Mouse, Fish, Monkey and Pig.

Quick Overview for HSPG antibody (Biotin) (ABIN6159600)

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Reactivity: Human, Cow, Mouse, Fish, Monkey, Pig

Host: Rat

Clonality: Monoclonal

Conjugate: This HSPG antibody is conjugated to Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: A7L6

Product Details anti-HSPG Antibody (Biotin)

Purpose: Rat Monoclonal anti-Heparan Sulfate Proteoglycan (A7L6), Biotin Conjugate

Characteristics: This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Murine EHS laminin preparation

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Breast carcinomas, squamous cell carcinomas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for HSPG

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Alternative Name: Heparan Sulfate Proteoglycan

Background: Heparan sulfate proteoglycan of basement membrane, HSPG, Hspg2, LG3 peptide, Perlecan, PLC, SJS1

Molecular Weight: >400 kDa

Gene ID: 3339, 562227

UniProt: P98160