CEACAM1 antibody (N-Term)

Catalog No. ABIN616012

This anti-CEACAM1 antibody is a Rabbit Polyclonal antibody detecting CEACAM1 in WB, FACS and EIA. Suitable for Human.

Quick Overview for CEACAM1 antibody (N-Term) (ABIN616012)

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEACAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Product Details anti-CEACAM1 Antibody

Binding Specificity: N-Term

Specificity: This antibody will detect recombinant Human soluble CEACAM-1. It shows cross-reactivity with several other CEACAM familiy members. There is no cross-reactivity with Human CEACAM-8.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein A Affinity Chromatography

Immunogen: Highly pure recombinant Human soluble CEACAM-1 (N-term)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS pH 7.4, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CEACAM1

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Alternative Name: CD66a / CEACAM1

Background: Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1, also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa's 35-142). The three soluble forms also contain the first two C2-type Ig like domains (aa's 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57 % aa identical to human CEACAM1, in the V-type Ig-like domain, they are 58 % and 56 % aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.Synonyms: BGP, BGP1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1

Gene ID: 634

NCBI Accession: NP_001703

UniProt: P13688