G-CSF antibody (CF®488A)

Catalog No. ABIN6161607

This Mouse Monoclonal antibody specifically detects G-CSF in IF, FACS and IHC (f). It exhibits reactivity toward Human and Macaque.

Quick Overview for G-CSF antibody (CF®488A) (ABIN6161607)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human, Macaque

Host: Mouse

Clonality: Monoclonal

Conjugate: This G-CSF antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CSF3-900

Product Details anti-G-CSF Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-Granulocyte Colony Stimulating Factor (G-CSF) (CSF3) (CSF3/900), CF488A Conjugate

Characteristics: This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF's, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF's share 73 % sequence identity at the amino acid level. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human recombinant full-length CSF3 protein

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HL60 cells. Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: Granulocyte Colony Stimulating Factor (G-CSF)

Background: Pluripoietin, Filgrastim, Lenograstim, CSF3OS, G-CSF, chromosome 17 open reading frame 33 (C17orf33), colony stimulating factor 3 (granulocyte)

Molecular Weight: 19 kDa

Gene ID: 1440, 2233

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization