Desmoglein 2 antibody (Biotin)

Catalog No. ABIN6162222

This Mouse Monoclonal antibody specifically detects Desmoglein 2 in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Desmoglein 2 antibody (Biotin) (ABIN6162222)

Target: Desmoglein 2 (DSG2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 6D8

Product Details anti-Desmoglein 2 Antibody (Biotin)

Purpose: Mouse Monoclonal anti-Desmoglein-2 (6D8), Biotin Conjugate

Characteristics: This antibody recognizes the extracellular domain of human desmoglein-2 (DSG2), a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. It was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length DSG2 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431, HepG2 or K562 cells. Heart or Colon

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Desmoglein 2

Target: Desmoglein 2 (DSG2)

Alternative Name: Desmoglein-2

Background: ARVC10, ARVD10, Cadherin family member 5, CDHF5, CMD1BB, Desmoglein-2, DSG2, HDGC, Human Desmoglein Colon

Molecular Weight: ~165 kDa

Gene ID: 1829, 412597

UniProt: Q14126

Pathways: Cell-Cell Junction Organization