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GNRHR antibody (N-Term)

This Mouse Monoclonal antibody specifically detects GNRHR in IF, FACS and IHC (f). It exhibits reactivity toward Human and Rat.
Catalog No. ABIN6163446

Quick Overview for GNRHR antibody (N-Term) (ABIN6163446)

Target

See all GNRHR Antibodies
GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Reactivity

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Human, Rat

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This GNRHR antibody is un-conjugated

Application

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

F1G4
  • Binding Specificity

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    AA 1-29, N-Term

    Purpose

    Mouse Monoclonal anti-GnRH Receptor (LHRH Receptor) (F1G4), Purified, BSA-free

    Characteristics

    This antibody recognizes an epitope on the extracellular domain of gonadotropin releasing hormone (GnRH) receptor or luteinizing hormone receptor (LHCGR). Lutropin (also designated luteinizing hormone) plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Gonadotropin (also designated choriogonadotropin) production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone/choriogonadotropin receptor. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). GnRH influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of GnRH on breast carcinoma correlates in part to the degree of tumor differentiation. GnRH-positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women. GnRH is present in luteal and granulosa cells as well as in ovarian cell membrane preparations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Purification

    Purified

    Immunogen

    A synthetic peptide aa 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) from the N-terminal of human GnRH receptor.

    Isotype

    IgG1 kappa
  • Application Notes

    Immunohistology formalin-fixed 1-2 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Predicted to react with pig or rabbit, others not known
    • Optimal dilution for a specific application should be determined by user

    Comment

    T47D cells. Pituitary gland, ovarian or breast cancers.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS (no BSA, no azide)

    Preservative

    Azide free
  • Target

    GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

    Alternative Name

    GnRH Receptor (LHRH Receptor)

    Molecular Weight

    54-60 kDa
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