Growth Hormone 1 antibody (AA 58-187) (CF®647)

Catalog No. ABIN6163607

This anti-Growth Hormone 1 antibody is a Mouse Monoclonal antibody detecting Growth Hormone 1 in IHC, IF and FACS. Suitable for Human.

Quick Overview for Growth Hormone 1 antibody (AA 58-187) (CF®647) (ABIN6163607)

Target: Growth Hormone 1 (GH1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Growth Hormone 1 antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: GH-1371

Product Details anti-Growth Hormone 1 Antibody (CF®647)

Binding Specificity: AA 58-187

Purpose: Mouse Monoclonal anti-Growth Hormone (GH/1371), CF647 Conjugate

Characteristics: Pituitary growth hormone (GH) plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Anti-GH is a useful marker in classification of pituitary tumors and the study of pituitary disease (acromegaly). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant human Growth Hormone (GH) fragment (aa58-187) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Pituitary cells or Pituitary

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Alternative Name: Growth Hormone

Background: GH, GH-N, GH1, GHN, Growth hormone 1, Growth hormone, Growth hormone, pituitary, HG1, hGH-N, IGHD1B, Pituitary growth hormone, RNGHGP, Somatotropin

Molecular Weight: 20 kDa

Gene ID: 2688, 655229

UniProt: P01241

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process