Involucrin antibody (CF®647)

Catalog No. ABIN6164785

The Mouse Monoclonal anti-Involucrin antibody has been validated for IF, FACS, ISt and IHC (f). It is suitable to detect Involucrin in samples from Human.

Quick Overview for Involucrin antibody (CF®647) (ABIN6164785)

Target: Involucrin (IVL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is conjugated to CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: IVRN-827

Product Details anti-Involucrin Antibody (CF®647)

Purpose: Mouse Monoclonal anti-Involucrin (IVRN/827), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified involucrin from human keratinocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37C
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Note than enzyme digestion is better than citrate buffer based epitope unmasking
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: Involucrin

Background: IVL

Molecular Weight: 66-170 kDa

Gene ID: 3713, 516439

UniProt: P07476