Thymidine Phosphorylase antibody (CF®405S)

Catalog No. ABIN6165119

This anti-Thymidine Phosphorylase antibody is a Mouse Monoclonal antibody detecting Thymidine Phosphorylase in WB, IHC and IP. Suitable for Human, Rat and Mouse.

Quick Overview for Thymidine Phosphorylase antibody (CF®405S) (ABIN6165119)

Target: Thymidine Phosphorylase (TYMP)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thymidine Phosphorylase antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: P-GF-44C

Product Details anti-Thymidine Phosphorylase Antibody (CF®405S)

Purpose: Mouse Monoclonal anti-Thymidine Phosphorylase / PD-ECGF (P-GF.44C), CF405S Conjugate

Characteristics: This antibody recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Optimal dilution for a specific application should be determined by user

Comment:

HUVEC cells. Breast, Bladder, Lung or Kaposi tumors

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Alternative Name: Thymidine Phosphorylase / PD-ECGF

Background: ECGF, ECGF1, Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PD-ECGF, PDECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine phosphorylase, TP, Tymp

Molecular Weight: 55 kDa

Gene ID: 1890, 180903

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2