MVP antibody (CF®405S)

Catalog No. ABIN6165347

This anti-MVP antibody is a Mouse Monoclonal antibody detecting MVP in FACS, IF and IHC (f). Suitable for Human.

Quick Overview for MVP antibody (CF®405S) (ABIN6165347)

Target: MVP (Major Vault Protein (MVP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MVP antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 1032

Product Details anti-MVP Antibody (CF®405S)

Purpose: Mouse Monoclonal anti-Major Vault Protein (MVP) (1032), CF405S Conjugate

Characteristics: This antibody recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Proteins precipitated from human breast cancer MCF-7 cells

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7/HeLa, breast tumors

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Background: Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1

Molecular Weight: 104-110 kDa

Gene ID: 9961, 632177

UniProt: Q14764